Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea

AXENIC CULTURE AND CRYOPRESERVATION OF GIARDIA LAMBLIA ISOLATED IN IRAN

Giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. The cysts were harvested and purified using the sucrose gradient method. IS The excystation of G. lamblia was obtained by treating them with HCI (0.01N)', then they were transferred on to modified TYI-S-33 medium. Out of 55 stool samples 17 cases were excysted with a rate of ...

axenic culture and cryopreservation of giardia lamblia isolated in iran

giardia lamblia cysts were isolated from fecal samples of 55 symptomatic and asymptomatic patients with giardiasis. the cysts were harvested and purified using the sucrose gradient method. is the excystation of g. lamblia was obtained by treating them with hci (0.01n)', then they were transferred on to modified tyi-s-33 medium. out of 55 stool samples 17 cases were excysted with a rate of ...

Characterization of proteins from the cytoskeleton of Giardia lamblia.

Proteins from the axonemes and disc cytoskeleton of Giardia lamblia have been examined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. In addition to tubulin and the 30 X 10(3) molecular weight disc protein, at least 18 minor components copurify with the two major proteins in Triton-insoluble structures. The most prominent minor bands have the apparent molecular weights of 110 X ...

Giardia Lamblia.

in vitro effects of metronidazole and albendazole on giardia lamblia isolated from iranian patients

background : the aim of the present study was to evaluate the effects of metronidazole and albendazole against clinical isolates of giardia lamblia in vitro. methods : from all human samples of containing cysts, 10 isolates were successfully excysted in vitro . trophozites viability was assessed by eosine 0.1% and cultured axenically in tyi-s-33 modified medium supplemented with heat inactivate...